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Briefly, cultures were grown in regular BHI until mid-exponential phase (OD 600nm ~0.4) and homologized in Tris-HCl buffer, 50 mM pH 7.4 containing 10% glycerol using a glass bead beater as described (Wen et al., 2006)

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Experimental site, duration, and growing season A pot experiment was conducted during the Rabi (cool, winter) crop season of 2024–2025 under natural environmental conditions in a protected wirehouse in the research area of the Department of Agronomy, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Punjab, Pakistan (29.39° N, 71.66° E)

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