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7/ While the increased osmotic load of sorbitol was initially felt to be a part of the pathogenesis of diabetic complications, it's less clear that this plays a role. Instead, something else

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snRNA-seq and analysis For isolation of nuclei from gWAT, fresh adipose tissue was minced in nucleus preparation buffer (10 mM HEPES, 1.5 mM MgCl 2 , 10 mM KCl, 250 mM sucrose, 0.001% NP-40) with 0.2 U µl Roche Protector RNase inhibitor followed by dounce homogenization