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Artificial Sequence FR forward primer for site directed mutagenesis to generate sHASEGP fusion with kappa leader with P 38 as the first sHASEGP amino acid after the kappa leader 34 ttcagagcac ctcctgttat tccaaatg 28 35 23 DNA Artificial Sequence Asp reverse primer for site directed mutagenesis to generate sHASEGP fusion with kappa leader with Asp as the last Kappa leader amino acid before L 36 or F 38 of PH-20 35 gtcaccagtg gaacctggaa ccc 23 36 24 DNA Artificial Sequence Gly reverse primer for site directed mutagenesis to generate sHASEGP fusion with kappa leader with Gly as the last Kappa leader amino acid before L 36 or F 38 of PH-20 36 accagtggaa cctggaaccc agag 24 37 30 DNA Artificial Sequence Forward primer for first fragment of kappa leader 37 gagacagaca cactcctgct atgggtactg 30 38 30 DNA Artificial Sequence Reverse primer for first fragment of kappa leader 38 cccagagcag cagtacccat agcaggagtg 30 39 30 DNA Artificial Sequence Forward primer for second fragment of kappa leader 39 ggtactgctg ctctgggttc caggttccac 30 40 27 DNA Artificial Sequence Reverse primer for second fragment of kappa leader 40 gcgtcaccag tggaacctgg aacccag 27 41 30 DNA Artificial Sequence Nhe Forward primer for kappa leader 41 attgctagca tggagacaga cacactcctg 30 42 28 DNA Artificial Sequence EcoR1 reverse primer for kappa leader 42 aattgaattc gtcaccagtg gaacctgg 28 43 21 PRT Artificial Sequence Mouse IgK-chain leader sequence 43 44 30 DNA Artificial Sequence K-leader SPE 1 FORWARD Primer 44 actcactagt gctagcatgg agacagacac 30 45 30 DNA Artificial Sequence K-leader MLU1 REV primer 45 aattacgcgt gaattcgtca ccagtggaac 30 46 462 PRT Artificial Sequence Kappa leader fusion protein with sHASEGP with F 38 as the first amino acid of the putative mature secreted sHASEGP (up to P478) 46 47 40 DNA Artificial Sequence 3' Primer BAM REV sHASEGP with GPI anchor up to L 509 including STOP 47 aattggatcc ctacagaaga aatgataaga aacaaaatac 40 48 1449 DNA