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2.7 Preparation of mitochondria and assay of enzyme activity The enzyme activity of three respiratory-chain complexes was quantified in the mitochondria of L-02 hepatocytes treated with CGA and in untreated cells, as previously described ( 2.8 Quantitative analysis of oxidized and reduced forms of glutathione (GSH) To detect GSH and GSSG (glutathione disulfide), two 200 μL sample solutions were prepared

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