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In order to measure GSH, 0.5 mL of total blood was treated at 4°C with 0.5 mL ice-cold perchloric acid (6%), containing 1 mM bathophenanthroline disulphonic acid (BPDS)

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mPEG–PLGA NPs containing DETA NONOate mPEG–PLGA and PLGA NPs encapsulating DETA NONOate were prepared using the modified double emulsion (water-in-oil-in-water) method, as previously reported (Figure 2)