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Even other, non-desired proteins, that have Histidine side chains will flow through because they do not have as many as the desired, tagged protein, which would have about 6 adjacent Histidine residues

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[DOI] [PMC free article] [PubMed] [Google Scholar] 36.Goyal P., Choi J.J., Pinheiro L.C., Schenck E.J., Chen R., Jabri A., Satlin M.J., Campion T.R., Jr., Nahid M., Ringel J.B., et al